Alexa Fluor® 647 anti-mouse/rat CD61 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse, Rat

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 104325 and 104326).

Application References (PubMed link indicates BioLegend citation)

Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block)
Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
Ashkar S, et al. 2000. Science 287:860. (Block)
Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ)
Carlson TR, et al. 2008.135:2193. PubMed
Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed

Product Citations

Yu L, et al. 2020. Cell Stem Cell. 28(3):550-567.e12. PubMed
Asselin-Labat M, et al. 2011. Mol Cell Biol. 31:4609. PubMed
Elsarraj H, et al. 2013. J Cell Sci. 126:2446. PubMed
Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
Baasch S, et al. 2021. Cell. . PubMed
Brait VH, et al. 2019. Circ Res. 124:279. PubMed
Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
Laidlaw T, et al. 2012. Blood. 119:3790. PubMed
Feng Q et al. 2018. The Journal of physiology. 597(3):781-798 . PubMed

RRID

AB_2249337 (BioLegend Cat. No. 104313) AB_2234024 (BioLegend Cat. No. 104314)

Antigen Details

Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand/Receptor: Fibrinogen, von Willebrand factor, fibronectin
Cell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Phillips DR, et al. 1991. Cell. 65:359.
3. Felding-Habermann B, et al. 1993. Curr?Opinion Cell Biol. 5:864.
Gene ID: 16416 View all products for this Gene ID 29302 View all products for this Gene ID
UniProt: View information about CD61 on UniProt.org

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