Purified anti-mouse CD117 (c-Kit) Antibody
- 产品名称：
- Purified anti-mouse CD117 (c-Kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105801
- 产品应用：
- 105801
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Podd B, et al. 2006. J Immunol. 176:6532. PubMed
- Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Liu Y, et al. 2021. Nat Commun. 12:6323. PubMed
- Martin K,et al. 2017. J Immunol.. 10.4049/jimmunol.1601522. PubMed
- Batsivari A, et al. 2017. Stem Cell Reports. 8:1549. PubMed
- Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
- Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
- Wicki S, et al. 2018. Int J Mol Sci. 1.266666667. PubMed
- Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
- Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
- Morita S et al. 2017. Cell metabolism. 25(4):883-897 . PubMed
- Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
- Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
- Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
- Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed
- Bachelet I, et al. 2008. J Immunol. 180:6064. PubMed
- Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
- Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
- Russell AL, et al. 2021. Mol Ther Methods Clin Dev. 21:710. PubMed
- Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
- Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
- Coelho P, et al. 2015. Open Bio. 5: 150209. PubMed
- Gu Z, et al. 2019. Cancer Discov. 1.227777778. PubMed
- RRID
- AB_313210 (BioLegend Cat. No. 105801) AB_313211 (BioLegend Cat. No. 105802)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
