PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105823
- 产品应用：
- 105823
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Krauss J, et al. 2015. Proc Natl Acad Sci U S A. 112: 10455-10460. PubMed
- Chen S, et al. 2022. Cancer Discov. :. PubMed
- De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed
- Winn H 2010. Toxicol Sci. 113:207. PubMed
- Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
- Yoshimi A, et al. 2019. Nature. 574:273. PubMed
- Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
- Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
- Yamada D, et al. 2014. J Immunol. 192:4112. PubMed
- Chillo O, et al. 2016. Cell Rep. 16:2197-2207. PubMed
- Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
- Ledo JH, et al. 2020. PLoS One. e0237773:15. PubMed
- Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
- Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
- Ma Y, et al. 2009. Blood. 114:4402. PubMed
- Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
- Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
- Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
- Ledo JH, et al. 2020. Mol Psychiatry. . PubMed
- RRID
- AB_2131598 (BioLegend Cat. No. 105823) AB_2131597 (BioLegend Cat. No. 105824)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
