Pacific Blue™ anti-mouse CD117 (c-Kit) Antib
- 产品名称：
- Pacific Blue™ anti-mouse CD117 (c-Kit) Antib
- 产品类别：
- 抗体
- 产品编号：
- 105819
- 产品应用：
- 105819
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Dey S, et al. 2010. Mol Cell Biol. 30:2181. PubMed
- Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
- Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
- JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
- Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
- Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
- Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
- Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
- Yá?ez A et al. 2017. Immunity. 47(5):890-902 . PubMed
- RRID
- AB_493477 (BioLegend Cat. No. 105819) AB_493476 (BioLegend Cat. No. 105820)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
