FITC anti-mouse CD117 (c-Kit) Antibody
- 产品名称：
- FITC anti-mouse CD117 (c-Kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105805
- 产品应用：
- 105805
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed
- Chen W, et al. 2020. Cell Discov. 6:56. PubMed
- Adamiak M, et al. 2015. Leukemia. . PubMed
- Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
- Chen W, et al. 2020. Stem Cell Reports. 1.140972222. PubMed
- Timofeev O, et al. 2020. Cancer Res. 80:5231. PubMed
- Diesner S, et al. 2012. Immunol Lett. 141:210. PubMed
- Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
- Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
- Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
- Mitry MA, et al. 2020. American Journal of Physiology-Cell Physiology. 318(2):C380-C391. PubMed
- Chen W, et al. 2020. Cell Discov. 6:56. PubMed
- Gough D, et al. 2014. Blood. 124:2252. PubMed
- Brusilovsky M, et al. 2021. Nat Immunol. 22:1316. PubMed
- Liu B, et al. 2013. Development. 140:780. PubMed
- Cantor DJ et al. 2019. Cell reports. 26(1):108-118 . PubMed
- Lenkiewicz AM, et al. 2019. Stem Cell Rev. 0.896527778. PubMed
- Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
- Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
- Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed
- Zhang B, et al. 2021. Nature. 599:471. PubMed
- Jatho A, et al. 2022. Cells. 11:. PubMed
- RRID
- AB_313214 (BioLegend Cat. No. 105805) AB_313215 (BioLegend Cat. No. 105806)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
