Biotin anti-mouse CD117 (c-Kit) Antibody
- 产品名称：
- Biotin anti-mouse CD117 (c-Kit) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 105803
- 产品应用：
- 105803
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
- Kuroki S, et al. 2020. Stem Cell Reports. 0.919444444. PubMed
- Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
- Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
- Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
- Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
- Adam RC et al. 2018. Cell stem cell. 22(3):398-413 . PubMed
- Rai S, et al. 2014. PLoS One. 9:109441. PubMed
- Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
- Onodera T, et al. 2021. Immunity. 54:2385. PubMed
- Kandalla P, et al. 2016. J Exp Med. 213: 2269 - 2279. PubMed
- Fujii T, et al. 2021. Bone Res. 9:4. PubMed
- Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
- Laurin M, et al. 2019. Elife. 8. PubMed
- Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
- Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
- RRID
- AB_313212 (BioLegend Cat. No. 105803) AB_313213 (BioLegend Cat. No. 105804)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
