Alexa Fluor® 647 anti-mouse CD117 (c-Kit) Anti
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD117 (c-Kit) Anti
- 产品类别：
- 抗体
- 产品编号：
- 105817
- 产品应用：
- 105817
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?L volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Barkaway A, et al. 2021. Immunity. . PubMed
- Hodzic D, et al. 2022. PLoS Biol. 20:e3001811. PubMed
- Shade K, et al. 2015. J Exp Med. 212:457. PubMed
- Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
- Amouzegar A, et al. 2017. Stem Cells. 10.1002/stem.2611. PubMed
- Harhouri K, et al. 2010. Blood. 115:3843. PubMed
- Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
- Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
- Hoshii T, et al. 2018. Cell. 172:1007. PubMed
- Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
- RRID
- AB_493475 (BioLegend Cat. No. 105817) AB_493474 (BioLegend Cat. No. 105818)
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
