PE anti-mouse Ikaros Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1980	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

N-terminal half of mouse Ikaros fused to GST.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤0.015 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported references (for the relevant formats) include: immunofluorescence¹ and immunoprecipitation².

Does not cross-react with human.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes.? Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited.? In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References (PubMed link indicates BioLegend citation)

Cobb SB, et al. 2000. Genes & Dev. 14:2146. (IF)
Hahm K. et al. 1998. Genes Dev. 12:782. (IP)
Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed

Product Citations

Fistonich C, et al. 2018. J Exp Med. 215:2586. PubMed

RRID

AB_2561727 (BioLegend Cat. No. 653303) AB_2561728 (BioLegend Cat. No. 653304)

Antigen Details

Structure: Belongs to the Ikaros C2H2-type zinc-finger protein family. Contains 6 C2H2-type zinc fingers. 519 amino acids with predicted MW of 58 kD.
Distribution: Nucleus. Abundantly expressed in thymus, spleen and peripheral blood leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
Function: Transcription regulator of hematopoietic cell differentiation. Plays a role in the development of lymphocytes, B and T cells.
Interaction: Heterodimer with other IKAROS family members.
Cell Targets: Component of the chromatin-remodeling NuRD repressor complex which includes at least HDAC1, HDAC2, RBBP4, RBBP7, IKZF1, MTA2, MBD2, MBD3, MTA1L1, CHD3, and CHD4. Interacts directly with the CHD4 component of the NuRD complex.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. John LB and Ward AC, 2011. Mol. Immunol. 48:1272.
2. Merkenschlager M, 2010. FEBS Lett. 584:4910.
3. Thomas RM, et al. 2010. J. Biol. Chem. 285:2545.
4. Papathanasiou P, et al. 2009. Stem Cell. 27:3082.
5. Molnar A, et al. 1996. J. Immunol. 156:585.
Gene ID: 22778 View all products for this Gene ID
UniProt: View information about Ikaros on UniProt.org

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