上海细胞库
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|
| 规格 | 价格 | 库存 |
|---|---|---|
| 100µg | ￥ 5148 | 1 |
ICFC - Quality tested
ICC, WB, IP - Verified
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125??g per million cells in 100 ?L volume.?For immunocytochemistry, a concentration?of 2.5?μg/mL is recommended.?For western blotting, the suggested use of this reagent is 1.0??g/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 ?g/test. It is recommended that the reagent be titrated for optimal performance for each application.
It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1.0 - 10.0 ?g/mL?of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase.
For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.??
Cytoplasm, Ribosomes
