Purified anti-PLK-1 Phospho (Thr210) Antibody
- 产品名称：
- Purified anti-PLK-1 Phospho (Thr210) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 628901
- 产品应用：
- 628901
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Modified peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 ?g antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Molli PR, et al. 2010. J Cell Biol. 190:101. PubMed
- Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
- Product Citations
-
- Bucko PJ, et al. 2019. eLife. 0.333333333333333. PubMed
- Bucko PJ, et al. 2020. J Biol Chem. 295:13784. PubMed
- Yang S, et al. 2013. Cancer Res. 73:6722. PubMed
- Molli P, et al. 2010. J Cell Biol. 190:101. PubMed
- Tankiewicz‐Kwedlo A et al. 2017. British journal of pharmacology. 175(5):743-762 . PubMed
- RRID
- AB_439786 (BioLegend Cat. No. 628901) AB_439787 (BioLegend Cat. No. 628902)
- Structure
- Serine/Threonine family of protein kinases, cdc5/polo subfamily. Highly homologous to polo-like kinase (Drosophila). Contains two polo box domains. Predicted molecular weight 68 kD
- Distribution
-
Nuclear protein, highly expressed in placenta and colon
- Function
- Regulates cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase. May be required for cell division. Depletion of PLK-1 results in apoptosis
- Interaction
- Interacts with nuclear distribution gene C
- Modification
- Phosphorylation (Thr210 is major phosphorylation site in activated PLK-1 from mitotic cells)
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
1. Hamanaka R, et al. 1994. Cell Growth Differ. 5:249.
2. Lake RJ, et al. 1993. Mol. Cell. Biol. 13:7793.
3. Holtrich U, et al. 1994. P. Natl. Acad. Sci. USA 91:1736.
- Regulation
- Upregulated by growth stimulating agents. Regulated by cell cycle position (highest in G2/M phase and declines to nearly undetectable levels after mitosis and throughout G1)
- Gene ID
- 5347 View all products for this Gene ID
- UniProt
- View information about PLK-1 Phospho Thr210 on UniProt.org
