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Brilliant Violet 605™ anti-mouse CD279 (PD-1
产品名称：
Brilliant Violet 605™ anti-mouse CD279 (PD-1
产品类别：
抗体
产品编号：
135219
产品应用：
135219
[价格]
规格 价格 库存
125µL ￥ 2844 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
  3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
  4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
  5. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  6. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Wrzner K, et al. 2021. EBioMedicine. 63:103197. PubMed
  3. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  4. Verma S, et al. 2016. J Virol. 90: 650 - 658. PubMed
  5. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  6. Wang D, et al. 2018. Immunity. 48:659. PubMed
  7. Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed
  8. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  9. Baram T, et al. 2021. Cells. 10:. PubMed
  10. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  11. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  12. Lindenstr?m T et al. 2017. EBioMedicine. 27:27-39 . PubMed
  13. DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed
  14. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  15. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  16. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  17. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
  18. Marro BS, et al. 2019. Cell Rep. 29:3293. PubMed
  19. Levi J, et al. 2020. J Nucl Med. . PubMed
  20. Espinosa JR, et al. 2018. Front Immunol. 9:1371. PubMed
  21. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  22. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  23. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  24. Xu S, et al. 2021. Immunity. . PubMed
  25. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  26. Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed
  27. Vogel AB, et al. 2021. Nature. 592:283. PubMed
  28. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  29. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  30. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  31. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  32. Sandu I, et al. 2020. Cell Reports. 32(8):108078. PubMed
  33. Fu Y, et al. 2021. Front Cell Dev Biol. 9:689727. PubMed
  34. Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed
  35. Wen J, et al. 2020. Cell Rep. 31:107566. PubMed
  36. Lu YJ, et al. 2021. Cell Rep. 36:109696. PubMed
  37. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  38. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  39. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
RRID
AB_11125371 (BioLegend Cat. No. 135219) AB_2562616 (BioLegend Cat. No. 135220)

Antigen Details

Structure
A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function
Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor
B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, T cells
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

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