Alexa Fluor® 647 anti-mouse CD279 (PD-1) Antib
- 产品名称：
- Alexa Fluor® 647 anti-mouse CD279 (PD-1) Antib
- 产品类别：
- 抗体
- 产品编号：
- 135229
- 产品应用：
- 135229
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- PD-1 cDNA followed by PD-1-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3, in vivo blocking of PD-1 binding to its ligands2,3, and spatial biology (IBEX)5,6.
- Application References
(PubMed link indicates BioLegend citation) -
- Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
- Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
- Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
- Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Tata A, et al. 2021. Oncoimmunology. 10:1933808. PubMed
- Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
- Liu X, et al. 2020. Nature. . PubMed
- Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
- RRID
- AB_2566007 (BioLegend Cat. No. 135229) AB_2566008 (BioLegend Cat. No. 135230)
- Structure
- A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
- Distribution
-
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.
- Function
- Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
- Ligand/Receptor
- B7-H1 (PD-L1) and B7-DC (PD-L2)
- Cell Type
- B cells, T cells
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313
- Gene ID
- 18566 View all products for this Gene ID
- UniProt
- View information about CD279 on UniProt.org
