GoInVivo™ Purified anti-human CD274 (B7-H1, -上海雅吉生物科技有限公司

规格	价格	库存
5mg	￥ 11880	1

Product Details

Verified Reactivity

Human, African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full length human PD-L1

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <1.0 EU/mg of the protein. (<0.01 pg/?g of the protein) as determined by the LAL test.

Preparation

The GoInVivo? antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

GoInVivo? products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo? Webpage.

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region⁵.?Additional reported applications (for the relevant formats) include: blocking^1-3 and immunohistochemical staining of acetone-fixed frozen sections¹. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections. However, there are references cited that indicate that this clone has been used successfully in other FFPE applications¹.

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor? 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the?Alexa Fluor? 700?conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications⁷.

Application References (PubMed link indicates BioLegend citation)

Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
Haile, S et al. 2013. J. Immunol. 191:2829.
RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.

RRID

AB_2566287 (BioLegend Cat. No. 329728) AB_2566288 (BioLegend Cat. No. 329729) AB_2566289 (BioLegend Cat. No. 329730) AB_2566080 (BioLegend Cat. No. 329725) AB_2566081 (BioLegend Cat. No. 329726) AB_2566082 (BioLegend Cat. No. 329727)

Antigen Details

Distribution: T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells
Function: CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor: PD-1 (PDCD1)
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area: Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family: Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
Gene ID: 29126 View all products for this Gene ID
UniProt: View information about CD274 on UniProt.org

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