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Brilliant Violet 421™ anti-human CD274 (B7-H
产品名称：
Brilliant Violet 421™ anti-human CD274 (B7-H
产品类别：
抗体
产品编号：
329713
产品应用：
329713
[价格]
规格 价格 库存
25tests ￥ 2280 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human PD-L1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5.?Additional reported applications (for the relevant formats) include: blocking1-3 and immunohistochemical staining of acetone-fixed frozen sections1.?The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).?

It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor? 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the?Alexa Fluor? 700?conjugate of interest.

Clone 29E.2A3 does not work in Western blot applications7.

Application References

(PubMed link indicates BioLegend citation)
  1. Brown J, et al. 2003. J. Immunol. 170:1257. (FC, IHC, Block)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (Block)
  3. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (Block)
  4. Barsoum IB, et al. 2014. Cancer Res. 74:665. PubMed
  5. Haile, S et al. 2013. J. Immunol. 191:2829.
  6. RL M, et al. 2015. PNAS. 112:6506-6514. PubMed
  7. Mahoney KM, et al. 2015. Cancer Immunol. Res. 3:1308.
Product Citations
  1. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  2. Shenoy GN, et al. 2021. Clin Transl Immunology. 10:e1246. PubMed
  3. Nduom E, et al. 2016. Neuro Oncology. 18: 195 - 205. PubMed
  4. Mohan N, et al. 2022. Front Immunol. 13:918528. PubMed
  5. Ferrando–Martinez S, et al. 2019. JHEP Rep. 0.159722222. PubMed
  6. Perdigoto AL, et al. 2022. JCI Insight. 7:. PubMed
  7. Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
  8. Singh S, et al. 2022. Int J Mol Sci. 23:. PubMed
  9. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  10. Chakravorty S, et al. 2019. Cancer Res. 79:6010. PubMed
  11. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  12. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  13. AlHossiny M, et al. 2016. Cancer Res . 76: 3376 - 3386. PubMed
  14. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
  15. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  16. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  17. Maurer DM, et al. 2020. Cancer Immunol Res. 8:1554. PubMed
RRID
AB_10901164 (BioLegend Cat. No. 329713) AB_2563852 (BioLegend Cat. No. 329714)

Antigen Details

Distribution

T cells, B cells, NK cells, monocytes/macrophages, granulocytes and dendritic cells

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.

Gene ID
29126 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

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