PerCP/Cyanine5.5 anti-mouse CD335 (NKp46) Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD335 (NKp46) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 137609
- 产品应用：
- 137609
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- NKP46-IgG1 Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
- Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
- Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
- Product Citations
-
- Zhou J, et al. 2019. Immunity. 50:403. PubMed
- Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
- Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
- He L, et al. 2019. iScience. 0.790972222. PubMed
- Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
- Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
- Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
- Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
- Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
- Nabekura T, et al. 2020. Immunity. 96:52. PubMed
- RRID
- AB_10642684 (BioLegend Cat. No. 137609) AB_10641137 (BioLegend Cat. No. 137610)
- Structure
- Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
- Distribution
-
Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
- Function
- NK cells activation
- Ligand/Receptor
- Viral hemagglutinins, heparan sulfate proteoglycans
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
- Gene ID
- 17086 View all products for this Gene ID
- UniProt
- View information about CD335 on UniProt.org
