Brilliant Violet 421™ anti-mouse CD335 (NKp4
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD335 (NKp4
- 产品类别：
- 抗体
- 产品编号：
- 137611
- 产品应用：
- 137611
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- NKP46-IgG1 Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mL?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
- Application References
(PubMed link indicates BioLegend citation) -
- Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (FC, Activ)
- Walzer T, et al. 2007. Nat. Immunol. 8:1337. (FC, Activ)
- Guerriero JL, et al. 2011. J. Immunol. 186:3517. (IHC) PubMed
- Product Citations
-
- Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
- LaMarche NM, et al. 2020. Cell Metabolism. 32(2):243-258.e6. PubMed
- Huggins MA, et al. 2019. Cell Rep. 28:1729. PubMed
- Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
- Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
- Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
- Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
- Alikhanyan K, et al. 2020. Immun Inflamm Dis. 8:181. PubMed
- Shen JZ, et al. 2020. Cell. 184(2):352-369.e23. PubMed
- Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed
- Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed
- Goh W, et al. 2020. Cell Rep. 33:108285. PubMed
- Martomo SA, et al. 2021. Mol Cancer Ther. 1.074305556. PubMed
- Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
- Kissiov DU, et al. 2022. Elife. 11:. PubMed
- Wang X, et al. 2021. Cell. 184:5357. PubMed
- Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
- Wagner AK, et al. 2022. iScience. 25:105137. PubMed
- Lai NY, et al. 2020. Cell. 180:33:00. PubMed
- RRID
- AB_10915472 (BioLegend Cat. No. 137611) AB_2563104 (BioLegend Cat. No. 137612)
- Structure
- Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
- Distribution
-
Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
- Function
- NK cells activation
- Ligand/Receptor
- Viral hemagglutinins, heparan sulfate proteoglycans
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
- Gene ID
- 17086 View all products for this Gene ID
- UniProt
- View information about CD335 on UniProt.org
