PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)
- 产品名称：
- PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1)
- 产品类别：
- 抗体
- 产品编号：
- 142527
- 产品应用：
- 142527
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse mammary gland epithelial cell line NMuMG
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
- Application References
(PubMed link indicates BioLegend citation) -
- Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
- Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
- Li Q, et al. 2002. Cell 111:635. (IF, IHC)
- McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
- Product Citations
-
- Gaa R, et al. 2021. MAbs. 13:1978130. PubMed
- RRID
- AB_2566494 (BioLegend Cat. No. 142527) AB_2566495 (BioLegend Cat. No. 142528)
- Structure
- Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
- Function
- Cell adhesion, cell motility, cell migration, cell proliferation
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
- Gene ID
- 20969 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
