Brilliant Violet 421™ anti-mouse CD138 (Synd
- 产品名称：
- Brilliant Violet 421™ anti-mouse CD138 (Synd
- 产品类别：
- 抗体
- 产品编号：
- 142507
- 产品应用：
- 142507
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse mammary gland epithelial cell line NMuMG
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mL?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For flow cytometric staining using the ?l sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.
- Application References
(PubMed link indicates BioLegend citation) -
- Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
- Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
- Li Q, et al. 2002. Cell 111:635. (IF, IHC)
- McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
- Product Citations
-
- Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
- Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
- Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
- Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
- Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
- Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
- Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
- Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
- Pack AD, et al. 2021. Cell Reports. 36(8):109586. PubMed
- Huang L, et al. 2015. J Immunol. 194:283. PubMed
- Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
- Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
- Duan L, et al. 2021. Immunity. 54:2256. PubMed
- Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
- Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
- Mintz MA, et al. 2019. Immunity. 51:310. PubMed
- Ise W, et al. 2018. Immunity. 48:702. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
- Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
- Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
- Kr?utler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
- Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
- He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
- Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
- Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
- Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
- Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
- Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
- Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
- Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
- Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
- Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
- Viant C, et al. 2020. Cell. 183:1298. PubMed
- Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
- Nascimento DC, et al. 2021. Immunity. :. PubMed
- Chen PM, et al. 2022. Sci Adv. 8:eabo4271. PubMed
- Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
- Li X, et al. 2021. Front Immunol. 12:779560. PubMed
- Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
- RRID
- AB_11204257 (BioLegend Cat. No. 142507) AB_2565621 (BioLegend Cat. No. 142523) AB_11203544 (BioLegend Cat. No. 142508)
- Structure
- Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
- Function
- Cell adhesion, cell motility, cell migration, cell proliferation
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.
- Gene ID
- 20969 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org
