PerCP/Cyanine5.5 anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H.SW mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity¹, and immunohistochemical staining² of acetone-fixed frozen sections.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
Shao H, et al. 2005. J. Immunol. 175:1851. (FC)

Product Citations

Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed

RRID

AB_2750199 (BioLegend Cat. No. 114619) AB_2750200 (BioLegend Cat. No. 114620)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b/H-2D^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2K b,H-2D b on UniProt.org

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