PE anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H.SW mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity¹, and immunohistochemical staining² of acetone-fixed frozen sections.

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
Shao H, et al. 2005. J. Immunol. 175:1851. (FC)

Product Citations

Coulouarn C, et al. 2011. Carcinogenesis. 32:1434. PubMed
Wu MJ, et al. 2022. Cancer Discov. 12:812. PubMed
Liu X, et al. 2020. Nature. . PubMed
Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
Du L et al. 2019. Cell metabolism. 29(6):1363-1375 . PubMed
Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
Zhang Z, et al. 2020. Cancer Cell. 38(2):279-296. PubMed
Song TY, et al. 2021. Nat Commun. 12:7003. PubMed
Shou P, et al. 2016. Oncogene. 10.1038/onc.2016.128. PubMed
Bui J, et al. 2006. J Immunol. 176:905. PubMed

RRID

AB_313598 (BioLegend Cat. No. 114607) AB_313599 (BioLegend Cat. No. 114608)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2K^b/H-2D^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2K b,H-2D b on UniProt.org

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