Alexa Fluor® 647 anti-mouse H-2K
- 产品名称：
- Alexa Fluor® 647 anti-mouse H-2K
- 产品类别：
- 抗体
- 产品编号：
- 114612
- 产品应用：
- 114612
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H.SW mouse splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
- Application References
(PubMed link indicates BioLegend citation) -
- Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
- Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
- Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
- Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
- Product Citations
-
- Capietto AH, et al. 2020. J Exp Med. 217:00:00. PubMed
- Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed
- Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
- Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
- Li J, et al. 2020. Cancer Discov. . PubMed
- Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
- Li J, et al. 2018. Immunity. 49:178. PubMed
- Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
- Benci JL et al. 2019. Cell. 178(4):933-948 . PubMed
- Bui J, et al. 2006. J Immunol. 176:905. PubMed
- RRID
- AB_492931 (BioLegend Cat. No. 114612)
