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Purified anti-mouse H-2L
产品名称:
Purified anti-mouse H-2L
产品类别:
抗体
产品编号:
114502
产品应用:
114502
[价格]
规格 价格 库存
500µg ¥ 2964 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, immunoprecipitation3, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 114510).

Does not react with other haplotypes (e.g., k, f, p, r, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1980. J. Immunol. 125:2473.
  2. Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
  3. Marguet D, et al. 1999. Immunity 11:231.
  4. Bashuda H, et al. 1997. Transplantation 63:113.
  5. Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed
  6. Milstein O, et al. 2011. Blood 117:1042.
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Azuma T, et al. 2008. Blood. 111:3635. PubMed
  3. Frey BF, et al. 2018. J Immunol. 200:1853. PubMed
  4. Cheung KCP, et al. 2020. Nat Commun. 2.954861111. PubMed
RRID
AB_313583 (BioLegend Cat. No. 114502)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Ld/H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Ld, H-2Db on UniProt.org

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