PE anti-mouse H-2L-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H.SW mouse splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity¹, immunoprecipitation³, blocking of cell lysis, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 114510).

Does not react with other haplotypes (e.g., k, f, p, r, s).

Application References (PubMed link indicates BioLegend citation)

Ozato K, et al. 1980. J. Immunol. 125:2473.
Kafrouni MI, et al. 2001. J. Immunol. 167:1566.
Marguet D, et al. 1999. Immunity 11:231.
Bashuda H, et al. 1997. Transplantation 63:113.
Greeneltch KM, et al. 2007. Cancer Res.67:10406. PubMed
Milstein O, et al. 2011. Blood 117:1042.

Product Citations

Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
Greeneltch K, et al. 2007. Cancer Res. 67:10406. PubMed
Zheng Y, et al. 2022. Transl Res. :. PubMed

RRID

AB_313588 (BioLegend Cat. No. 114507)

Antigen Details

Structure: MHC class I
Distribution: Nucleated cells of the H-2L^d/H-2D^b haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt: View information about H-2Ld, H-2Db on UniProt.org

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