Ultra-LEAF™ Purified anti-human/mouse/rat MR-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2184	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Soluble complexes of MR1 ectodomain and B2M.

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
ELISA, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines^1-6, detection of folded MR1 conformers^3,7, and immunoprecipitation of MR1 from cell lysates⁷.

Application References (PubMed link indicates BioLegend citation)

Young MH, et al. 2013. PLoS One. 8:e53789. (Block)
Leeansyah E, et al. 2013. Blood 121:1124. (Block)
Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA)
Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC)
Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC)
Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC)
Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)

Product Citations

Sugimoto C, et al. 2022. Elife. 11:. PubMed

RRID

AB_2800999 (BioLegend Cat. No. 361109) AB_2801000 (BioLegend Cat. No. 361110)

Antigen Details

Structure: Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution: MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.
Function: Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor: α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14.
2. Young MH and Gapin L. 2013. Cell Res. 23:460.
3. Gold MC, et al. 2013. Mucosal Immunol. 6:35.
4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.
Gene ID: 3140 View all products for this Gene ID 15064 View all products for this Gene ID 25119 View all products for this Gene ID
UniProt: View information about MR1 on UniProt.org

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