Purified anti-human/mouse/rat MR1 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3516	1

Product Details

Verified Reactivity

Human, Mouse, Rat

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Soluble complexes of MR1 ectodomain and B2M.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
ELISA, IP, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines^1-6, detection of folded MR1 conformers^3,7, and immunoprecipitation of MR1 from cell lysates⁷.

Application References (PubMed link indicates BioLegend citation)

Young MH, et al. 2013. PLoS One. 8:e53789. (Block)
Leeansyah E, et al. 2013. Blood 121:1124. (Block)
Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA)
Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC)
Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC)
Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC)
Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)

Product Citations

Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
Berkson JD, et al. 2020. Immunohorizons. 4:14. PubMed
Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
Paquin–Proulx D, et al. 2018. PLoS Negl Trop Dis. 12:e0006154. PubMed
Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed
Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
Flament H, et al. 2021. Nat Immunol. 322:22. PubMed
Gherardin NA, et al. 2021. Cancer Immunol Res. 9:612. PubMed
Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed
Dias J, et al. 2017. Proc Natl Acad Sci U S A. 114:E5434. PubMed
Shaler C, et al. 2017. PloS Biol.. 15(6):e2001930. PubMed

RRID

AB_2562969 (BioLegend Cat. No. 361102)

Antigen Details

Structure: Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution: MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.
Function: Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor: α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14.
2. Young MH and Gapin L. 2013. Cell Res. 23:460.
3. Gold MC, et al. 2013. Mucosal Immunol. 6:35.
4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.
Gene ID: 3140 View all products for this Gene ID 15064 View all products for this Gene ID 25119 View all products for this Gene ID
UniProt: View information about MR1 on UniProt.org

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