Purified anti-human FOXP3 Antibody
- 产品名称：
- Purified anti-human FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 320201
- 产品应用：
- 320201
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus, Baboon, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length FOXP3 protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
ICFC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 ?g/ml in antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
- Yang ZZ, et al. 2006. Blood 107:3639. PubMed
- Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
- Groh V, et al. 2006. Nature Immunology 7:755. PubMed
- Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
- Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
- Gong G, et al. 2009. Blood 113:837. PubMed
- Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
- Long SA, et al. 2010. Diabetes. 59:407. PubMed
- Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
- Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
- Dupont G, et al. 2014. Cytokine. 69:146. PubMed
- Product Citations
-
- Passerini L, et al. 2008. Int Immunol. 1.125694444. PubMed
- Wang R, et al. 2009. Proc Natl Acad Sci U S A. 106:13439. PubMed
- Humblet-Baron S, et al. 2007. J Clin Invest. 117:407. PubMed
- Karandikar V 2008. Blood. 111:463. PubMed
- Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
- Kaffes I, et al. 2019. Oncoimmunology. 8:e1655360. PubMed
- Kegler A, et al. 2019. Oncoimmunology. 8:e1621676. PubMed
- Pan C, et al. 2021. Oncoimmunology. 10:1883890. PubMed
- RRID
- AB_430884 (BioLegend Cat. No. 320201) AB_430885 (BioLegend Cat. No. 320202)
- Structure
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hori S, et al. 2003. Science 299:1057.
- Regulation
- FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
- Gene ID
- 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
