PE anti-human FOXP3 Antibody
- 产品名称：
- PE anti-human FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 320207
- 产品应用：
- 320207
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2196 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus, Baboon, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length FOXP3 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 ?l staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
- Yang ZZ, et al. 2006. Blood 107:3639. PubMed
- Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
- Groh V, et al. 2006. Nature Immunology 7:755. PubMed
- Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
- Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
- Gong G, et al. 2009. Blood 113:837. PubMed
- Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
- Long SA, et al. 2010. Diabetes. 59:407. PubMed
- Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
- Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
- Dupont G, et al. 2014. Cytokine. 69:146. PubMed
- Product Citations
-
- Krebs FK, et al. 2021. Cancer Med. . PubMed
- Ariafar A, et al. 2020. Heliyon. 6:e05556. PubMed
- Zimmer N, et al. 2020. Cancers (Basel). 12:00. PubMed
- Eberhardt K, et al. 2015. Clin Infect Dis. 61: 1615 - 1623. PubMed
- Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
- Burbano YCB, et al. 2019. Rev Soc Bras Med Trop. 52:e20190101. PubMed
- Park K, et al. 2008. Cancer Res. 68:8400. PubMed
- Weiss E, et al. 2011. J Immunol. 187:1684. PubMed
- , et al. 2021. Eur J Immunol. 51:2708. PubMed
- Popmihajlov Z, Smith K 2008. PLoS One. 1.222916667. PubMed
- Voo K, et al. 2009. Proc Natl Acad Sci U S A. 106:4793. PubMed
- Cheng J, et al. 2022. iScience. 25:103588. PubMed
- Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
- Voo K, et al. 2013. J Immunol. 191:3641. PubMed
- Garg G, et al. 2012. J Immunol. 188:4644. PubMed
- Kuranda K, et al. 2018. J Clin Invest. 128:5267. PubMed
- RRID
- AB_492983 (BioLegend Cat. No. 320207) AB_492982 (BioLegend Cat. No. 320208)
- Structure
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hori S, et al. 2003. Science 299:1057.
- Regulation
- FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
- Gene ID
- 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
