Alexa Fluor® 647 anti-human FOXP3 Antibody
- 产品名称：
- Alexa Fluor® 647 anti-human FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 320213
- 产品应用：
- 320213
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1980 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus, Rhesus, Baboon, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length FOXP3 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The FOXP3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemical staining1 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The 259D antibody gives strong positivity on paraffin and frozen sections and the antibody stains some epithelial cells. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
- Yang ZZ, et al. 2006. Blood 107:3639. PubMed
- Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659. PubMed
- Groh V, et al. 2006. Nature Immunology 7:755. PubMed
- Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656.PubMEd
- Long SA, et al. 2008. J Autoimmun. 30:293. PubMed
- Gong G, et al. 2009. Blood 113:837. PubMed
- Long SA, et al. 2009. Eur J. Immunol. 39:612. PubMed
- Long SA, et al. 2010. Diabetes. 59:407. PubMed
- Ferraro A, et al. 2014. PNAS. 111:1111. PubMed
- Vudattu NK, et al. 2014. J Immunol. 193:587. PubMed
- Dupont G, et al. 2014. Cytokine. 69:146. PubMed
- Product Citations
-
- Long S, et al. 2009. Eur J Immunol. 39:612. PubMed
- Long S, Buckner J 2008. J Autoimmun. 30:293. PubMed
- Vudattu N, et al. 2014. J Immunol. 193:587. PubMed
- Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
- Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
- Demaret J, et al. 2015. J Leukoc Biol. 97:791. PubMed
- Presicce P, et al. 2011. PLoS One. 6:e28118. PubMed
- Gong G, et al. 2009. Blood. 113:837. PubMed
- Ibrahim OM, et al. 2021. Cancers (Basel). 13:. PubMed
- Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
- Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed
- Tran D, et al. 2007. Blood. 110:2983. PubMed
- Chen C, et al. 2012. J Immunol. 188:4278. PubMed
- Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed
- Dupont G, et al. 2014. Cytokine. 69:146. PubMed
- Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
- Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
- Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
- Long S, et al. 2010. Diabetes. 59:407. PubMed
- RRID
- AB_492985 (BioLegend Cat. No. 320213) AB_492984 (BioLegend Cat. No. 320214)
- Structure
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hori S, et al. 2003. Science 299:1057.
- Regulation
- FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
- Gene ID
- 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
