PE/Cyanine7 anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

SIINFEKL pulsed RMA-S cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. ?Additional reported applications (for relevant formats) include: Western Blotting^1,3, immunofluorescence microscopy^2,3, immunohistochemical staining of frozen tissue sections³ , and inhibition of T cell response to H-2K^b-SIINFEKL in vitro.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Mareeva T, et al. 2010. J. Immunol. Methods 353:78. (WB)
Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
Porgador A, et al. 1997. Immunity 6:715. (WB, IF, IHC)
Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.

Product Citations

Arora H, et al. 2019. Immunity. 50:418. PubMed
Liu X, et al. 2020. Nature. . PubMed
Baasch S, et al. 2021. Cell. . PubMed
Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
Gammon J, et al. 2015. J Control Release. 210: 169-178. PubMed

RRID

AB_11219193 (BioLegend Cat. No. 141607) AB_11218593 (BioLegend Cat. No. 141608)

Antigen Details

Structure: OVA peptide_257-264 and H-2K^b complex
Distribution: SIINFEKL bond H-2K^b bearing cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org

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