APC anti-mouse H-2K-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1356	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

SIINFEKL pulsed RMA-S cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
ICC?- Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. ?Additional reported applications (for relevant formats) include: Western Blotting^1,3, immunofluorescence microscopy^2,3, immunohistochemical staining of frozen tissue sections³ , and inhibition of T cell response to H-2K^b-SIINFEKL in vitro.

Application References (PubMed link indicates BioLegend citation)

Mareeva T, et al. 2010. J. Immunol. Methods 353:78. (WB)
Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
Porgador A, et al. 1997. Immunity 6:715. (WB, IF, IHC)
Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.

Product Citations

Sun L, et al. 2021. Cancer Cell. :. PubMed
Champagne J, et al. 2021. Mol Cell. 81:4709. PubMed
Yan J, et al. 2022. J Clin Invest. 132:. PubMed
Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
Zhang L, et al. 2021. J Immunother Cancer. 9:. PubMed
Yl?sm?ki E, et al. 2021. J Immunother Cancer. 9:. PubMed
Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
Pérez-Girón J, et al. 2015. J Vis Exp. 100: 52803. PubMed
Bartok O, et al. 2020. Nature. . PubMed
Fusciello M, et al. 2022. Mol Ther Oncolytics. 25:137. PubMed
Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
Cordeiro B, et al. 2020. Cell Reports. 31(5):107585. PubMed
Song TY, et al. 2021. Nat Commun. 12:7003. PubMed
Ahn J et al. 2018. Cancer cell. 33(5):862-873 . PubMed
Zhang Y, et al. 2022. Nat Commun. 13:4948. PubMed
Reguzova A, et al. 2020. Vaccines (Basel). 8:. PubMed
Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed

RRID

AB_11219402 (BioLegend Cat. No. 141605) AB_11219595 (BioLegend Cat. No. 141606)

Antigen Details

Structure: OVA peptide_257-264 and H-2K^b complex
Distribution: SIINFEKL bond H-2K^b bearing cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.
Gene ID: 14972 View all products for this Gene ID
UniProt: View information about H-2Kb bound to SIINFEKL on UniProt.org

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