Purified anti-mouse I-A-上海雅吉生物科技有限公司

规格	价格	库存
500µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

C3H.SW splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
IP, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity³, immunoprecipitation¹, immunohistochemical staining of acetone-fixed frozen sections^4,5 and acetone fixed epidermal sheets⁶.

Additional Product Notes

NULL

Application References (PubMed link indicates BioLegend citation)

Wall KA, et al. 1983. J. Immunol. 131:1056. (IP)
Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747.
Ozato K, et al. 1981. J. Immunol. 126:317.
Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC)
Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC)
Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed
Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed.
Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.

Product Citations

Wada N, et al. 2020. Mol Metab. 37:100988. PubMed
Motoyama S, et al. 2020. Cells. :9. PubMed
Meuter S, et al. 2007. Mol Cell Biol. 27:983. PubMed
Ryan S, et al. 2011. J Exp Med. 208:1041. PubMed
Sicking E, et al. 2012. J Am Soc Nephrol. 1.395138889. PubMed
Diao J, et al. 2018. J Immunol. 201:1306. PubMed

RRID

AB_313577 (BioLegend Cat. No. 114402)

Antigen Details

Structure: ? chain of I-A^b MHC class II
Distribution: B cell and activated T cells, APCs of H-2^b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14961 View all products for this Gene ID
UniProt: View information about I-Ab on UniProt.org

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