Alexa Fluor® 488 anti-mouse I-A
- 产品名称：
- Alexa Fluor® 488 anti-mouse I-A
- 产品类别：
- 抗体
- 产品编号：
- 114407
- 产品应用：
- 114407
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C3H.SW splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity3, immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections4,5 and acetone fixed epidermal sheets6.
- Application References
(PubMed link indicates BioLegend citation) -
- Wall KA, et al. 1983. J. Immunol. 131:1056. (IP)
- Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747.
- Ozato K, et al. 1981. J. Immunol. 126:317.
- Khanna A, et al. 2000. J. Immunol. 164:1346. (IHC)
- Nikolic B, et al. 1999. J. Immunol. 162:3402. (IHC)
- Meuter S, et al. 2007. Mol. Cell. Biol. 27:983. (IHC) PubMed
- Ryan AO, et al. 2011. J Exp Med. 208;1041. PubMed.
- Sicking EM, et al. 2012. J Am Soc Nephrol. 23:629. PubMed.
- RRID
- AB_493144 (BioLegend Cat. No. 114407)
- Structure
- ? chain of I-Ab MHC class II
- Distribution
-
B cell and activated T cells, APCs of H-2b mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
- Gene ID
- 14961 View all products for this Gene ID
- UniProt
- View information about I-Ab on UniProt.org
