PE anti-rat CD86 Antibody
- 产品名称：
- PE anti-rat CD86 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 200307
- 产品应用：
- 200307
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- HTLV-1-transformed Lewis-S1 rat T cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 200310).
- Application References
(PubMed link indicates BioLegend citation) -
- Maeda K, et al. 1997. Int. Immunol. 9:993. (IP, Block)
- Bluestone JA. 1995. Immunity 2:555. (IP, Block)
- Damoiseaux JG, et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
- Donius LR, et al. 2014. Immunobiology. 219:440. PubMed
- Product Citations
-
- Zonneveld-Huijssoon E, et al. 2012. Ann Rheum Dis. 71:1706. PubMed
- Li J, et al. 2022. Am J Transl Res. 14:5003. PubMed
- Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
- Wu H, et al. 2016. Sci Rep. 6: 24548. PubMed
- Martín-Fernández B, et al. 2016. PLoS One. 11: 0145946. PubMed
- Snell LM, et al. 2018. Immunity. 49:678. PubMed
- Li Y, et al. 2020. Front Immunol. 1.577083333. PubMed
- Lin ZH, et al. 2018. Front Cell Neurosci. 0.729861111. PubMed
- Wang N, et al. 2018. Exp Ther Med. 15:1906. PubMed
- Xin L, et al. 2022. Adv Healthc Mater. 11:e2201680. PubMed
- Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed
- RRID
- AB_313856 (BioLegend Cat. No. 200307) AB_313857 (BioLegend Cat. No. 200308)
- Structure
- Ig superfamily, 80 kD
- Distribution
-
Antigen presenting cells, activated B cells and T cells
- Function
- T and B cell costimulation
- Ligand/Receptor
- CD28, CD152 (CTLA-4)
- Cell Type
- B cells, T cells, Antigen-presenting cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Ann. Rev. Immunol. 20:29.
- Gene ID
- 56822 View all products for this Gene ID
- UniProt
- View information about CD86 on UniProt.org
