Ultra-LEAF™ Purified anti-human CD154 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2712	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, ICC?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3?and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Brams P,?et al.?2001.?Int. Immunopharmacol.?1:277. (Block, IF)
Rushworth SA,?et al.?2002.?Transplantation?73:635. (Block)
Berner B,?et al.?2000.?Ann. Rheum. Dis.?59:190. (IF)
Nordstr?m T,?et al.?2006.?J. Leukocyte Biol.?79:319. (Block)
Zhang AL,?et al.2007.?Blood?doi:10.1182/blood-2007-02-076364. (Block)?PubMed
Kuchen S,?et al.?2007.?J. Immunol.?179:5886.
Matus-Nicodermos R,?et al.?2011.?J. Immunol.?186:2164.?PubMed
Peterson VM,?et al.?2017.?Nat. Biotechnol.?35:936. (PG)

RRID

AB_11150400 (BioLegend Cat. No. 310827) AB_11150401 (BioLegend Cat. No. 310828)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

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