Purified anti-human CD154 (Maxpar® Ready) Anti-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2748	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3?and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Brams P,?et al.?2001.?Int. Immunopharmacol.?1:277. (Block, IF)
Rushworth SA,?et al.?2002.?Transplantation?73:635. (Block)
Berner B,?et al.?2000.?Ann. Rheum. Dis.?59:190. (IF)
Nordstr?m T,?et al.?2006.?J. Leukocyte Biol.?79:319. (Block)
Zhang AL,?et al.2007.?Blood?doi:10.1182/blood-2007-02-076364. (Block)?PubMed
Kuchen S,?et al.?2007.?J. Immunol.?179:5886.
Matus-Nicodermos R,?et al.?2011.?J. Immunol.?186:2164.?PubMed
Peterson VM,?et al.?2017.?Nat. Biotechnol.?35:936. (PG)

RRID

AB_2563722 (BioLegend Cat. No. 310835)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

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