Pacific Blue™ anti-human CD154 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3180	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy^1,3?and blocking of T cell-dependent B cell differentiation^1,2,4,5. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References (PubMed link indicates BioLegend citation)

Brams P,?et al.?2001.?Int. Immunopharmacol.?1:277. (Block, IF)
Rushworth SA,?et al.?2002.?Transplantation?73:635. (Block)
Berner B,?et al.?2000.?Ann. Rheum. Dis.?59:190. (IF)
Nordstr?m T,?et al.?2006.?J. Leukocyte Biol.?79:319. (Block)
Zhang AL,?et al.2007.?Blood?doi:10.1182/blood-2007-02-076364. (Block)?PubMed
Kuchen S,?et al.?2007.?J. Immunol.?179:5886.
Matus-Nicodermos R,?et al.?2011.?J. Immunol.?186:2164.?PubMed
Peterson VM,?et al.?2017.?Nat. Biotechnol.?35:936. (PG)

Product Citations

Savage TM, et al. 2018. JCI Insight. 3:. PubMed
Schoenbrunn A, et al. 2012. J Immunol. 189:5985. PubMed
Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed
Li T, et al. 2022. J Clin Invest. 132:. PubMed

RRID

AB_830699 (BioLegend Cat. No. 310820)

Antigen Details

Structure: TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution: Activated T cells
Function: B cell costimulation
Ligand/Receptor: CD40
Cell Type: T cells, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.
Gene ID: 959 View all products for this Gene ID
UniProt: View information about CD154 on UniProt.org

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