Alexa Fluor® 488 anti-human CD51/61 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3180	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Chicken, Cynomolgus, Rabbit

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation⁵, immunohistochemical staining of acetone-fixed frozen tissue sections⁵, immunofluorescence microscopy⁵, and blocking of cell adhesion^4,6. The LEAF? Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 304414).

Application References (PubMed link indicates BioLegend citation)

Knapp WB, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Horton M, et al. 1991. Exp. Cell Res. 195:368.
Takahashi R, et al. 1999. Blood 93:1951. (Block)
Davies J, et al. 1989. J. Cell Biol. 109:1817. (IF, IHC, IP)
Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709. (FC, Block)
Barau A, et al. 2010. J. Ultrasound Med. 29:173. PubMed

Product Citations

Del Mistro G, et al. 2022. Cell Death Dis. 13:54. PubMed

RRID

AB_528843 (BioLegend Cat. No. 304408)

Antigen Details

Structure: Integrin, CD51/CD61 heterodimer, 24 kD
Distribution: Platelets, B subset, activated T cells, monocytes/macrophages, osteoblasts, endothelial cells, melanoma
Function: Adhesion, bone metabolism and apoptosis
Ligand/Receptor: Vitronectin, von Willebrand factor, fibrinogen, thrombospondin, osteopontin, denatured collagen
Cell Type: B cells, Endothelial cells, Macrophages, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Angiogenesis, Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Davies J, et al. 1989. J. Cell Biol. 109:1817.
2. Nesbitt S, et al. 1993. J. Biol. Chem. 268:16737.
Gene ID: 3685 View all products for this Gene ID 3690 View all products for this Gene ID
UniProt: View information about CD51 CD61 on UniProt.org

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