PerCP/Cyanine5.5 anti-mouse/human Helios Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2748	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Helios peptide (aa 51-107)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear? 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
Angin M, et al. 2014. PLoS One. 9:86920. PubMed
Bedke T, et al. 2014. Immunol Cell Biol. PubMed
Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed

Product Citations

Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
Stathopoulou C, et al. 2020. Immunity. 49(2):247-263.e7.. PubMed
Pagel J, et al. 2020. Front Immunol. 11:565257. PubMed
Yang L, et al. 2020. Genes Dis. 7:128. PubMed
Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed

RRID

AB_2561639 (BioLegend Cat. No. 137229) AB_2561640 (BioLegend Cat. No. 137230)

Antigen Details

Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al.? 1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt: View information about Helios on UniProt.org

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