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首页 > 抗体蛋白 > 抗体
Pacific Blue™ anti-mouse/human Helios Antibo
产品名称：
Pacific Blue™ anti-mouse/human Helios Antibo
产品类别：
抗体
产品编号：
137210
产品应用：
137210
[价格]
规格 价格 库存
25tests ￥ 2532 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Helios peptide (aa 51-107)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.
For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear? 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
  2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
  3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
  4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
  5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
  6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
  7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations
  1. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  2. Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed
  3. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  4. Schoenbrunn A, et al. 2012. J Immunol. 189:5985. PubMed
  5. Hope C, et al. 2014. Kidney Int. 86:175. PubMed
  6. Merwe M, et al. 2013. J Immunol. 191:5746. PubMed
  7. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  8. Kreiser S, et al. 2014. Immunobiology. 220:270. PubMed
  9. Singh K, et al. 2015. Sci Rep. 14:7767. PubMed
  10. Bouvy A, et al. 2014. Nephrol Dial Transplant. 29:1587. PubMed
  11. Yero A, et al. 2019. J Virol. 93:e00371-19. PubMed
  12. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  13. Baptista A, et al. 2014. Elife. 3:4433. PubMed
  14. Esfahani K, et al. 2019. Nat Commun. 3.688888889. PubMed
  15. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  16. Dhuban K, et al. 2015. J Immunol. 194:3687. PubMed
  17. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  18. Hu M, et al. 2019. Nat Commun. 10:3031. PubMed
  19. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  20. Serre K, et al. 2011. PLoS One. 6:e20731. PubMed
  21. Yang YH, et al. 2020. Cell Reports. 30(5):1515-1529.e4. PubMed
  22. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  23. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  24. Chopp LB, et al. 2020. Immunity. 53(6):1182-1201.e8. PubMed
  25. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  26. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  27. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_10575625 (BioLegend Cat. No. 137210) AB_10690535 (BioLegend Cat. No. 137220)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al.? 1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org

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