热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Alexa Fluor® 647 anti-mouse/human Helios Antib
产品名称：
Alexa Fluor® 647 anti-mouse/human Helios Antib
产品类别：
抗体
产品编号：
137208
产品应用：
137208
[价格]
规格 价格 库存
25tests ￥ 2472 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Helios peptide (aa 51-107)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol.
For flow cytometric staining, the suggested use of this reagent is 5 ?l per 106 cells in 100 ?l volume or 5 ?l per 100 ?l whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear? 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
  2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
  3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
  4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
  5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
  6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
  7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations
  1. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  2. F?hse L, et al. 2013. J Immunol. 191:2384. PubMed
  3. Schoenbrunn A, et al. 2012. J Immunol. 189:5985. PubMed
  4. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  5. He X, et al. 2017. Sci Rep. 7:43003. PubMed
  6. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  7. Lam AJ, et al. 2021. Cell Reports. 36(5):109494. PubMed
  8. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  9. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  10. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  11. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  12. Allenspach E, et al. 2017. J Allergy Clin Immunol. 10.1016/j.jaci.2017.02.017. PubMed
  13. Paiva R, et al. 2013. Proc Natl Acad Sci U S A. 110:6494. PubMed
  14. Landman S, et al. 2018. J Immunol Res. 2018:4973964. PubMed
  15. Wainwright D, et al. 2011. Neuro Oncology. 1.45. PubMed
  16. Benner M, et al. 2020. Cell Rep. 32:108204. PubMed
  17. Chopp LB, et al. 2020. Immunity. 53(6):1182-1201.e8. PubMed
RRID
AB_10552902 (BioLegend Cat. No. 137208) AB_10660750 (BioLegend Cat. No. 137218)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al.? 1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线