Alexa Fluor® 488 anti-mouse/human Helios Antib-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2472	1

Product Details

Verified Reactivity

Mouse, Human

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Helios peptide (aa 51-107)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per 10⁶ cells in 100 ?l volume or 5 ?l per 100 ?l whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear? 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References (PubMed link indicates BioLegend citation)

Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
Angin M, et al. 2014. PLoS One. 9:86920. PubMed
Bedke T, et al. 2014. Immunol Cell Biol. PubMed
Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed

Product Citations

Kaser T, et al. 2014. Dev Comp Immunol. 49:323. PubMed
Ma C, et al. 2018. Science. 360:eaan5931. PubMed
Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
Lee CG, et al. 2019. JCI Insight. 4:5. PubMed
Ritacco C, et al. 2021. Bone Marrow Transplant. 56:1828. PubMed
Wainwright D, et al. 2011. Neuro Oncology. 1.45. PubMed
Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
Olson KE, et al. 2021. EBioMedicine. 67:103380. PubMed

RRID

AB_10645334 (BioLegend Cat. No. 137213) AB_10661895 (BioLegend Cat. No. 137223)

Antigen Details

Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes.
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Kelly CM, et al.? 1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt: View information about Helios on UniProt.org

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