Purified anti-TIF1β (KAP-1, TRIM28) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, store between 2°C and 8°C.

Application

WB - Quality tested
ICC - Verified
ChIP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 ?g per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence¹, Western blotting¹ and chromatin immunoprecipitation¹.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

This clone may produce increased background by western blot when used at concentrations exceeding 0.1 ?g/mL.

Application References (PubMed link indicates BioLegend citation)

Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)

Product Citations

Ouyang C, et al. 2022. Int J Biol Sci. 18:2655. PubMed

RRID

AB_2209724 (BioLegend Cat. No. 619301) AB_2209723 (BioLegend Cat. No. 619302)

Antigen Details

Structure

Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD

Distribution

Nuclear, associates with specific chromatin regions

Function

Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression

Biology Area

Cell Biology, Immunology, Transcription Factors

Molecular Family

Protein Kinases/Phosphatase

Antigen References

1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366.
2. Schultz DC, et al. 2002. Genes Dev. 16:919.
3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859.
4. Friedman JR, et al. 1996. Genes Dev. 10:2067.
5. Messerschmidt DM, et al. 2012. Science 335:1499.
6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

Gene ID