Spark NIR™ 685 anti-human FOXP3 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1980	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Cynomolgus, Rhesus, Pigtailed Macaque

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Full-length FOXP3 protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark NIR? 685 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR? 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections¹ and formalin-fixed paraffin-embedded sections^1,8,19-20, and Western blotting¹. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References (PubMed link indicates BioLegend citation)

Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC)
Yang ZZ, et al. 2006. Blood 107:3639.
Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed
Bollyky PL, et al. 2007. J. Immunol. 179:744.
Bell MP, et al. 2007. J. Immunol. 179:1893.
Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed
Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed
Pillai V,et al. 2008. Blood 111:463. PubMed
Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed
Zonios DI, et al. 2008.Blood112:287. PubMed
Kavanagh B, et al. 2008. Blood. PubMed
Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed
Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed
Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed
Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed
Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed
Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed
Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed

RRID

AB_2890753 (BioLegend Cat. No. 320129) AB_2890753 (BioLegend Cat. No. 320130)

Antigen Details

Structure: Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution: Nuclear; expressed in T regulatory cells
Function: Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction: Interacts with DNA
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Hori S, et al. 2003. Science 299:1057.
2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.
Regulation: FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.
Gene ID: 50943 View all products for this Gene ID
UniProt: View information about FOXP3 on UniProt.org

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