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Brilliant Violet 421™ anti-human FOXP3 Antib
产品名称：
Brilliant Violet 421™ anti-human FOXP3 Antib
产品类别：
抗体
产品编号：
320123
产品应用：
320123
[价格]
规格 价格 库存
25tests ￥ 2580 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus, Pigtailed Macaque
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length FOXP3 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤5 ?l per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1,8,19-20, and Western blotting1. The binding of 206D to FOXP3 can be partially blocked by 259D, but 206D does not show significant blocking effect on 259D binding.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2005. Eur. J. Immunol. 35:1681.(IHC)
  2. Yang ZZ, et al. 2006. Blood 107:3639.
  3. Liu W, et al. 2006. J. Exp. Med. 203:1701.PubMed
  4. Bollyky PL, et al. 2007. J. Immunol. 179:744.
  5. Bell MP, et al. 2007. J. Immunol. 179:1893.
  6. Tran DQ, et al. 2007. Blood doi:10.1182/blood-2007-06-094656. PubMed
  7. Gao Q,et al.2007.J Clin Oncol.25:2586.(IHC) PubMed
  8. Pillai V,et al. 2008. Blood 111:463. PubMed
  9. Zheng Y, et al. 2008. J. Immunol. 181:1683. PubMed
  10. Zonios DI, et al. 2008.Blood112:287. PubMed
  11. Kavanagh B, et al. 2008. Blood. PubMed
  12. Nevala WK, et al. 2009. Clin Cancer Res. 15:1931. PubMed
  13. Grant J, et al. 2009. Cytometry B Clin Cytom. 76:69. PubMed
  14. Nigam P, et al. 2010. J. Immunol. 184:1690. PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (ICFC) PubMed
  16. Hartigan-O'Connor DJ,et al.2007.J Exp Med.204:2679. PubMed
  17. Raghaven S, et al. 2009. Ann Rheum Dis. 68:1908. PubMed
  18. Hodi FS, et al. 2014. Cancer Immunol Res. 2:632.(IHC) PubMed
  19. Sziros E, et al. 2015. Clin Cancer Res. 21:2840.(IHC) PubMed
Product Citations
  1. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  2. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  3. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  4. Turner JS, et al. 2021. Nature. 596:109. PubMed
  5. Au L, et al. 2021. Cancer Cell. 39:1497. PubMed
  6. Castillo J, et al. 2019. Cancer Res. 79:3916. PubMed
  7. Fenton TM, et al. 2020. Immunity. 52(3):557-570. PubMed
  8. Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed
  9. Ye LL, et al. 2020. Annals of Translational Medicine. 8(24):1647. PubMed
  10. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  11. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  12. Roshani Asl E, et al. 2021. J Cell Physiol. 236:7071. PubMed
  13. Bossini-Castillo L, et al. 2022. Cell Genom. 2:None. PubMed
  14. Byford ET, et al. 2018. PLoS One. 13:e0190468. PubMed
  15. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  16. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  17. Li Y, et al. 2022. Bioengineered. 13:4730. PubMed
  18. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID
AB_2561338 (BioLegend Cat. No. 320123) AB_2565972 (BioLegend Cat. No. 320124)

Antigen Details

Structure
Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
Distribution

Nuclear; expressed in T regulatory cells

Function
Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
Interaction
Interacts with DNA
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hori S, et al. 2003. Science 299:1057.
2. Gandhi R, et al. 2010. Nat. Immunol. 11:846.

Regulation
FOXP3 is present at high levels in T regulatory cells, it can also be induced by T cell activation.
Gene ID
50943 View all products for this Gene ID
UniProt
View information about FOXP3 on UniProt.org

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