Ultra-LEAF™ Purified anti-mouse CD88 (C5aR) -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3240	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

mC5aR transfected cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: blocking of C5a binding.

Application References (PubMed link indicates BioLegend citation)

Godau J, et al. 2004. J. Immunol. 173:3437.
Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
Kim SH, et al. 2013. Int Immunol. 25:623. PubMed

Product Citations

Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed

RRID

AB_2819875 (BioLegend Cat. No. 135815) AB_2819876 (BioLegend Cat. No. 135816)

Antigen Details

Structure: Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as G_i, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA₂, among other molecules.
Bioactivity: Chemotaxis, degranulation.
Cell Type: Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
Gene ID: 12273 View all products for this Gene ID
UniProt: View information about CD88 on UniProt.org

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