PE/Cyanine7 anti-mouse CD88 (C5aR) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

mC5aR transfected cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume.? It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications for the relevant formats include: blocking of C5a binding.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Godau J, et al. 2004. J. Immunol. 173:3437.
Shushakova N, et al. 2002. J. Clin. Invest. 110:1823.
Kim SH, et al. 2013. Int Immunol. 25:623. PubMed

Product Citations

Sharma S, et al. 2015. J Immunol. 194:5529. PubMed

RRID

AB_10900077 (BioLegend Cat. No. 135809) AB_10900812 (BioLegend Cat. No. 135810)

Antigen Details

Structure: Single chain protein with seven membrane-spanning regions, 43 kD.
Distribution: Neutrophils, eosinophils, monocytes, glial cells, cerebellar granule cells, cardiomyocytes, and vascular endothelial cells.
Function: After C5a binding, the CD88 signal results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction: CD88 is coupled to heterotrimeric G proteins such as G_i, The signal transduced by CD88 results in the activation of PLCβ, PI-3 kinase, and PLA₂, among other molecules.
Bioactivity: Chemotaxis, degranulation.
Cell Type: Dendritic cells, Endothelial cells, Eosinophils, Monocytes, Neutrophils
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Schraufstatter IU, et al. 2009. J. Immunol. 182:3827.
2. Ippel JH, et al. 2009. J. Biol. Chem. 284:12363.
3. Monk PN, et al. 2007. Br. J. Pharmacol. 152:429.
4. Griffin RS, et al. 2007. J. Neurosci. 27:8699.
5. Lee H, et al. 2006. Nat. Biotechnol. 24:1279.
6. Buck E and Wells JA, et al. 2005. P. Natl. Acad. Sci. USA 102:2719.
Gene ID: 12273 View all products for this Gene ID
UniProt: View information about CD88 on UniProt.org

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