Direct-Blot™ HRP anti-RNA Polymerase II RPB3
- 产品名称:
- Direct-Blot™ HRP anti-RNA Polymerase II RPB3
- 产品类别:
- 抗体
- 产品编号:
- 665005
- 产品应用:
- 665005
- Verified Reactivity
- Yeast (Saccharomyces cerevisiae)
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.
Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.
- Application References
(PubMed link indicates BioLegend citation) -
- Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
- RRID
- AB_2566702 (BioLegend Cat. No. 665005)