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Direct-Blot™ HRP anti-RNA Polymerase II RPB3
产品名称:
Direct-Blot™ HRP anti-RNA Polymerase II RPB3
产品类别:
抗体
产品编号:
665005
产品应用:
665005
[价格]
规格 价格 库存
100µL ¥ 4080 1

产品详情

Product Details

Verified Reactivity
Yeast (Saccharomyces cerevisiae)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This clone is yeast RPB3-specific and should not be used to detect mammalian RPB3.

Additional reported applications (for the relevant formats) include: chromatin immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Svetlov V, et al. 1998. J. Biol. Chem. 273:10827.
RRID
AB_2566702 (BioLegend Cat. No. 665005)

Antigen Details

Structure
RPB3 is a 45 kD subunit of the yeast RNA polymerase II.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
854791 View all products for this Gene ID
UniProt
View information about RNA Polymerase II RPB3 on UniProt.org

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