Alexa Fluor® 647 anti-Pax-5 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2112	1

Product Details

Verified Reactivity

Human, Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Recombinant mouse Pax-5 protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our?True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

The monoclonal 1H9 antibody?recognizes both mouse and human Pax5.

Additional reported applications (for the relevant formats) include: Western blotting^1,2, immunohistochemical staining of formalin-fixed sections, and spatial biology (IBEX)^4,5.

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Kallies A, et al. 2007. Immunity 26:555. (WB)
McManus S, et al. 2011. EMBO J. 30:2388. (WB)
Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2562424 (BioLegend Cat. No. 649703) AB_2562425 (BioLegend Cat. No. 649704)

Antigen Details

Structure: 391 amino acids with predicted molecular weight of 42 kD
Distribution: Nucleus
Function: Plays an important role in B-cell differentiation as well as neural development and spermatogenesis; involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene
Interaction: Interacts with DAXX; binds DNA as a monomer, binds TLE4
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Liao F, et al. 1992. J. Immunol. 148:2909.
2. Nutt SL, et al. 1997. Genes Dev. 11:476.
3. Nera KP, et al. 2006. Immunity 24:283.
4. Fuxa M, et al. 2007. Immunity 178:3031.
Gene ID: 5079 View all products for this Gene ID
UniProt: View information about Pax-5 on UniProt.org

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