Purified anti-complement C3/C3b/iC3b/C3d Antibody
- 产品名称：
- Purified anti-complement C3/C3b/iC3b/C3d Antibody
- 产品类别：
- 抗体
- 产品编号：
- 846302
- 产品应用：
- 846302
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human iC3b coupled to sepharose 4B
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Direct ELISA?- Verified
FC, ICC, ELISA Detection - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 ?g per ml.?For Direct ELISA, the suggested use of this reagent is 0.01 - 2.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: flow cytometry3, immunofluorescent staining2, and ELISA Detection1.
This antibody recognizes human C3, C3b, iC3b, and C3d.
- Application References
(PubMed link indicates BioLegend citation) -
- Tosic L, et al. 1989. J. Immunol. Methods 120:241. (ELISA)
- Kennedy AD, et al. 2003. Blood 101:1071. (IF)
- Lindorfer MA, et al. 2010. Blood 115:2283. (FC)
- Product Citations
-
- Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
- Kim H, et al. 2022. Nat Commun. 13:6581. PubMed
- RRID
- AB_2572128 (BioLegend Cat. No. 846302)
- Structure
- C3 is a 185 kD glycoprotein that is cleaved to generate a 176 kD protein fragment, C3b. iC3b is proteolytically derived from C3b and exists as a mixture of 176 and 174 kD proteins. C3d is a 33.8 kD single chain proteolytic fragment of iC3b.
- Distribution
-
Extracellular, plasma membrane, and serum.
- Function
- C3b is involved in all three complement pathways and is essential for effective complement activation and presentation of antigens to cells of the adaptive immune system. iC3b and C3d are less active than C3b, but the target-bound protein can expand target-specific B-cell and T-cell populations by interacting with lymphoid and phagocytic cells.
- Interaction
- Immune cells interact with C3b, iC3b and C3d using CR1, CR2/CR3 and CR2, respectively.
- Ligand/Receptor
- C3b can bind to Factor B, Factor P, Factor H, Factor I, C5, DAF (CD55), MCP (CD46), and CR1 (CD35). iC3b interacts with CR2 (CD21) and CR3 (CD11b/CD18). C3d binds to CR2.
- Biology Area
- Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Antigen References
-
1. Lambris JD, et al. 1988. Immunol. Today 9:387-93.
2. Dempsey PW, et al. 1996. Science 271:348-50.
- Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3/C3b/iC3b/C3d on UniProt.org
