PE anti-mouse ESAM Antibody
- 产品名称：
- PE anti-mouse ESAM Antibody
- 产品类别：
- 抗体
- 产品编号：
- 136203
- 产品应用：
- 136203
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bEND.3 endothelial cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application References
(PubMed link indicates BioLegend citation) -
- Nasdala I, et al. 2002. J. Bio. Chem. 277:16294
- Product Citations
-
- Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
- De Simone G, et al. 2021. Immunity. :. PubMed
- Barbet G, et al. 2018. Immunity. 48:584. PubMed
- Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
- Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
- RRID
- AB_1953300 (BioLegend Cat. No. 136203) AB_1953301 (BioLegend Cat. No. 136204)
- Structure
- A 55-kD membrane protein composed of two extracellular Ig domains, a single transmembrane domain, and a cytoplasmic domain.
- Distribution
-
Expressed at endothelial junctions, platelets, and murine hematopoietic stem cells.
- Function
- It impacts vascular permeability and extravasation process. ESAM expression is involved in hematopoietic stem cell (HSC) activity.
- Cell Type
- Endothelial cells, Hematopoietic stem and progenitors, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Wegmann F, et al. 2004. Exp Cell Res. 300:121
2. Ooi LAG, et al. 2008. Stem Cells. 27:653
3. Yokota T, et al. 2009. Blood 113(13):2871. Phosphate-buffered solution pH 7.2 containing 0.09% sodium azide.
- Gene ID
- 69524 View all products for this Gene ID
- UniProt
- View information about ESAM on UniProt.org
